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32961.氨基胍对非酒精性脂肪性肝炎大鼠TGF-β1及MMP2、TIMP2表达的影响
[医药制造业] [2014-02-25]
目的:研究实验性非酒精性脂肪性肝炎(NASH)大鼠转化生长因子-β1(TGF-β1)、基质金属蛋白酶-2(MMP2)、基质金属蛋白酶抑制因子-2(TIMP2)表达情况及氨基胍(AG)的干预作用。方法:SD大鼠28只,随机分为3组,模型组(M组)、氨基胍组(A组)、正常组(N组)。检测各组大鼠肝湿重、血糖(GLU)、谷丙转氨酶(ALT)、三酰甘油(TG)、胆固醇(CHO)及透明质酸(HA)含量。免疫组化法判断各组大鼠肝脏病理学变化。竞争性ELISA测定血清糖基化终产物(AGEs)及TGF-β1含量。Western-blot检测肝组织TGF-β1及MMP2、TIMP2蛋白表达情况。结果:M组大鼠肝...
关键词：氨基胍;非酒精性脂肪性肝炎;糖基化终产物;转化生长因子-β1;基质金属蛋白酶-2;基质金属蛋白酶抑制因子-2
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32962.补中益气丸补气疗效的谱-效关系研究
[医药制造业] [2014-02-25]
目的:探讨补中益气丸高效液相色谱(HPLC)指纹图谱与其补气作用的关联性,确立其补气药效成分。方法:建立补中益气丸的HPLC-二极管阵列检测器(DAD)和HPLC-蒸发光散射检测器(ELSD)指纹图谱;用控食法和疲劳法联合复制小鼠气虚模型,测定小鼠体质量、脏器指数、廓清指数和矫正廓清指数;用灰关联度分析方法研究其谱-效关系。结果:HPLC-DAD指纹图谱中各峰显示橙皮苷(峰号11)、芒柄花素(峰号19)对补气作用有较大贡献。HPLC-ELSD指纹图谱中各峰显示橙皮苷(峰号4)对补气作用有较大贡献。结论:补中益气丸对模型小鼠气虚症状有明显改善作用;HPLC-DAD和HPLC-ELSD指纹图谱均与...
关键词：补中益气丸;指纹图谱;补气疗效;灰关联度;谱-效关系
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32963.HPLC法测定奥拉西坦原料药中的有关物质
[医药制造业] [2014-02-25]
目的:建立测定奥拉西坦原料药有关物质的方法。方法:采用高效液相色谱法。色谱柱为月旭XB-C18,流动相为0.01mol/L磷酸二氢钾溶液(pH 3.0),流速为0.8 ml/min,检测波长为214 nm。结果:在选定的色谱条件下,主峰和相邻杂质峰能较好地分离;奥拉西坦检测质量浓度线性范围为0.210 4~0.631 2μg/m(lr=0.999 7,n=5),检测限为0.95 ng。结论:本方法准确、简便,专属性强,灵敏度高,可用于奥拉西坦原料药的质量控制。
关键词：高效液相色谱法;奥拉西坦原料药;有关物质
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32964.糖尿病脂肪肝患者血浆CHGA与ATGL水平相关性研究
[医药制造业] [2014-02-25]
目的探讨2型糖尿病(T2DM)合并非酒精性脂肪肝(NAFLD)患者血浆嗜铬蛋白A(CHGA)与脂肪组织三酰甘油脂肪酶(ATGL)的相关性。方法采用酶联免疫吸附法检测T2DM合并NAFLD组(A组,74例)、T2DM不合并NAFLD组(B组,76例)和正常对照组(NC组,75例)血浆CHGA和ATGL水平,分析各代谢指标与CHGA、ATGL的相关性,回归分析T2DM合并NAFLD的影响因素。结果血浆CHGA(μg/L)水平在A组(83.15±9.46)、B组(70.90±2.75)均高于NC组(46.74±8.15),A组高于B组(均P
关键词：糖尿病,2型;脂肪肝;脂肪酸类,非酯化;嗜铬蛋白A;三酰甘油脂肪酶
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32965.变形链球菌耐氟菌ffh基因siRNA干扰序列的筛选鉴定
[医药制造业] [2014-02-25]
目的筛选鉴定变形链球菌耐氟菌(UA159-FR)ffh基因的siRNA干扰序列。方法设计合成4段21bp的核酸[1],利用电穿孔方法转入UA159-FR使其与ffh基因序列靶向位点结合。通过RT-PCR和Real-time PCR技术验证靶向沉默ffh基因的效果,筛选出最佳siRNA干扰序列。结果倒置显微镜观察细菌电转效果正常。转染12h内除siRNA1外其他3对siRNA都可以抑制ffh基因的表达,而在转染超过12h达到24h后,只有siRNA2可以更稳定的抑制ffh基因的表达。结论 siRNA干扰技术可以有效的沉默UA159-FR的ffh基因,siRNA2在沉默过程中效果较稳定。
关键词：siRNA;ffh基因;变形链球菌耐氟菌
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32966.两种药物对甲硝唑治疗无效人芽囊原虫病的效果观察
[医药制造业] [2014-02-25]
目的观察红霉素、白头翁汤、红霉素加白头翁汤对甲硝唑治疗无效的人芽囊原虫病的作用效果。方法体外实验:体外培养人芽囊原虫,分别加不同浓度上述药物并于24 h和72 h后对虫体进行计数从而判断药物体外作用效果。临床效果观察:将112例确诊为人芽囊原虫病且甲硝唑治疗2个疗程后未能阴转的患者分3组,分别予红霉素、白头翁汤、红霉素加白头翁汤治疗,连服10日为1个疗程.治疗前后均行粪检查找人芽囊原虫。另设30例病人为对照组。结果体外实验:红霉素组≥80μg/ml组72h后虫体全部死亡,最适杀虫浓度为20μg/ml;白头翁汤组6400μg/ml组24 h、72h后虫体全部死亡,最适杀虫浓度为1600μg/ml...
关键词：红霉素;白头翁汤;人芽囊原虫;药物效果
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32967.波棱瓜子提取物在大鼠体内分布的初步研究
[医药制造业] [2014-02-25]
目的:初步了解波棱瓜子提取物在大鼠体内的组织分布情况,为进一步确定其药效成分及作用机制奠定基础。方法:大鼠单次灌胃给予一定剂量的波棱瓜子乙酸乙酯提取部位,分别在给药后1 h、2 h处死并解剖大鼠,取心、肝、脾、肺、肾、脑六个组织;采用高效液相色谱法(HPLC)对比空白组织、给药组织及体外样品,对各组织中移行成分进行确定,了解波棱瓜子提取物中各成分在大鼠体内的分布状态。结果:大鼠给药后,肺出现了4个移行成分,心、肝组织中出现了两个移行成分,脾、肾组织在2 h出现了一个移行成分,脑组织中未有移行成分出现。结论:波棱瓜子提取物口服给药后,多种成分均有吸收。给药后1 h~2 h间,各脏器分布广泛,其中...
关键词：波棱瓜子;体内分布;HPLC
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32968.全球骨质疏松症药物市场报告（2014-2018年）
[医药制造业] [2014-02-24]
Osteoporosis is a disease that reduces the mass and density of a bone, resulting in an increased risk of fracture. In this disease, the risk of fracture increases due to the permeability and fragility of the bones. Often there are no symptoms observed until the first fracture occurs. Some of the risk factors responsible for osteoporosis include aging, low body weight, low sex hormones or menopause, and smoking. Osteoporosis affects men and women of all races. It is estimated that, globally, 1 in 5 men and 1 in 3 women are at risk of an osteoporotic fracture.
关键词：骨质疏松症；药物；市场
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32969.全球性肠道疾病测试市场报告（2014-2018年）
[医药制造业] [2014-02-23]
The Global Enteric Disease Testing market is one of the fastest growing IVD markets. Over the years, the market has experienced remarkable growth, and is expected to witness an improvement in its growth rate and post a CAGR of 2.83 percent during the period 2013-2018. The majority of the growth stems from the Americas, followed by the EMEA and APAC regions. Although a majority of the growth is derived from the Bacterial Disease Testing segment, other segments such as Parasitic and Viral Disease Testing are expected to make significant contributions to the overall market revenue in the future.
关键词：肠道疾病；病毒；细菌；寄生虫
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32970.免疫反应的调节雄激素剥夺和放射治疗前列腺癌的治疗
[医药制造业] [2014-02-22]
Although the combination of radiation therapy and androgen deprivation therapy (ADT) is initially effective in many patients, biochemical failure rates of 20at 5-years to 50at 10-years have been reported, highlighting the need for improved treatments, particularly for men with high- risk prostate cancer. ADT in the neo-adjuvant setting is used to reduce tumor volume and improve the response to radiation. Additionally, ADT causes infiltration of lymphocytes into the prostate. B cell infiltrates may promote prostate cancer progression and development of castration resistant prostate cancer by the production of inflammatory cytokines and skewing CD4+ T cell responses towards Th2. We hypothesized that depletion of B cells at the time of castration would improve tumor control. Our results demonstrate that the depletion of B cells at the time of castration improves tumor latency.
关键词：治疗技术；前列腺；放疗和雄激素剥夺疗法(ADT)
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