Objective:p53,a tumor suppressor gene,is one of the hotspots in the world of the biomedical field.Mutation of p53 gene,which is found in approximately 50％ of human cancers,is a key event in carcinogenesis.This project aims to investigate the new characteristics of two p53 mutants,p53-W248 and p53-H175,in MCF-7 cells,so as to provide the experimental basis for understanding the functional alternations of mutant p53.Methods:In this study,MCF-7 cells transfected with p53-H175 or p53-W248 plasmids were used as experimental group and the MCF-7 cells transfected wild type p53 plasmid were used as control group.Then the biological effects at the cellular level were investigated using 3-(4.5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay,flow cytometry analysis and cell scratch test.Results:MTT assay showed that p53-W248 might promote cell proliferation in MCF-7 cells.The results of flow cytometry indicated that no significant effect on cell cycle progression and cell apoptosis by p53-H175 or p53-W248 in cells.The cell scratch test showed that p53-H175 could increase the ability of cell migration.Conclusion:p53-H175 could lead to the promotion of tumor cell migration,while p53-W248 may promote tumor cell proliferation,p53-H175 and p53-W248 might have acquired some new characteristics of oncogenes.

关键词：细胞增殖；细胞周期；细胞迁移；生物医药

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The U.S. Renewable Fuel Standard (RFS2) is a strategic response to concerns under the Energy Independence and Security Act (EISA) of 2007 about petroleum fuel supplies and environmental sustainability (EPA 2012). RFS2 regulations mandate specific amounts of renewable fuels to be blended into gasoline and diesel. The renewable fuels also need to emit fewer greenhouse gases than the displaced petroleum. In 2012, the target is for 9of all fuel will be from renewable sources, or a minimum of 15.2 billion gallons of renewable fuel. By 2022, the target is for 36 billion gallons of renewable fuel to be blended into gasoline and diesel and of that amount, 16 billion gallons derived from cellulosic biofuels. While some advanced biofuel will be refined from forest, crop, and urban residues, this paper focuses on the patterns of farmers choices regarding dedicated perennial lignocellulosic energy crops. Throughout this paper we use the term perennial as shorthand for warm season grasses such as those in the genus Panicum or Miscanthus, or short rotation tree crops such as those in the genus Populus or Salix. We focus on choices about these perennial crops because two thirds of the mandated advanced biofuels are expected to be converted at biorefineries from perennials (USDA 2010). RFS2 establishes the goals, but not mechanisms for achieving them. Mechanisms and incentives, however, are embedded in other legislation and programs. For example, the 2008 Farm Bill created the Biomass Crop Assistance Program (BCAP) that is designed to entice farmers to plant and deliver biomass feedstock to biorefineries and electrical plants. The effectiveness of this new program, however, has yet to be fully explored (Aguilar et al. 2011). In 2011, the program received over 40 project area proposals, exceeding the available funding, suggesting increasing adoption, or at least exceeding expectations of policymakers.

关键词：生物燃料；生物质；农业分析；生物能源

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Eastern Bering Sea shelf walleye pollock (Theragra chalcogramma) abundance and distribution in midwater were assessed between 5 June and 7 August 2010 using acoustic-trawl techniques aboard the NOAA ship Oscar Dyson. The survey also assessed walleye pollock in the Cape Navarin area of Russia. Results showed that ocean surface temperatures were warmer in 2010 than in recent years (1.8DG 12.3DGC in 2010 vs. 0.7DG 8.3DGC in 2 0 and 0.9DG 8.9DGC in 2009). The majority of the pollock biomass in the U.S. Exclusive Economic Zone (EEZ) was located to the south and west of St. Matthew Island between the 100 m and 200 m isobaths. Estimated pollock abundance in midwater (between 16 m from the surface and 3 m off bottom) in the U.S. EEZ portion of the Bering Sea shelf was high compared to recent surveys. The 2010 pollock biomass estimate in the U.S. EEZ was 2.323 million metric tons (t). The 2009 biomass was 0.924 million t and the estimated 2008 biomass was 0.997 million t. East of 170DG W, the predominant length mode was 40 cm and most ages ranged between 3 and 6 years. In the U.S. west of 170DG W (87.4of total biomass), dominant modal lengths were 26, 16, and 39 cm, corresponding to pollock aged 2, 1, and 4, respectively. In Russia (5.3of total biomass), modal lengths and ages were generally similar to those in the U.S. west of 170DG W. Vertical distribution analyses indicated that 85of adult biomass was within 40 m of the seafloor. Juveniles were found both near the seafloor and higher in the water column; 65of juvenile biomass was within 40 m of the seafloor while 13was within 45 m of the surface. Finally, the first field tests of a non-extractive stereo camera-trawl system (Cam-Trawl) were successfully completed during the 2010 survey, and the multifrequency euphausiid backscatter index of abundance was successfully computed, indicating a reduced abundance of euphausiids compared with that in summer 2009.

关键词：狭鳕；海洋设备；生物质能；数据分析

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This document contains 9 papers that study algorithmic development and preliminary testing of an ecodrive application; comparative analysis of car-following models for emissions estimations; comparisons of locomotive emissions during dynamometer versus rail yard engine load tests; the ratio of in-vehicle to near-vehicle exposure concentrations of airborne fine particles; and, emission benefits of commercial vehicle lane management strategies. This issue also studies environmentally conscious highway design for vertical grades; a fuel consumption prediction model for buses; discretionary passenger vehicle idling behaviors by season and trip stage; and, a statistical study of variables associated with particulate matter exposure levels at bus shelters.

关键词：空气污染；空气质量；运输研究

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关键词：空气污染；空气质量；技术支持文件模型

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The U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/NFO) operates the Nevada National Security Site (NNSS) and North Las Vegas Facility (NLVF). From 1951 through 1992, the NNSS was the continental testing location for U.S. nuclear weapons. The release of radionuclides from NNSS activities has been monitored since the initiation of atmospheric testing. Limitation to underground detonations after 1962 greatly reduced radiation exposure to the public surrounding the NNSS. After nuclear testing ended in 1992, NNSS radiation monitoring focused on detecting airborne radionuclides from historically contaminated soils. These radionuclides are derived from re-suspension of soil (primarily by wind) and emission of tritium-contaminated soil moisture through evapotranspiration. Low amounts of legacy-related tritium are also emitted to air at the NLVF, an NNSS support complex in North Las Vegas. To protect the public from harmful levels of man-made radiation, the Clean Air Act, National Emission Standards for Hazardous Air Pollutants (NESHAP) (Title 40 Code of Federal Regulations (CFR) Part 61 Subpart H) (CFR 2010a) limits the release of radioactivity from a U.S. Department of Energy (DOE) facility to that which would cause 10 millirem per year (mrem/yr) effective dose equivalent to any member of the public. This limit does not include radiation unrelated to NNSS activities. Unrelated doses could come from naturally occurring radioactive elements, from sources such as medically or commercially used radionuclides, or from sources outside of the United States, such as the damaged Fukushima nuclear power plant in Japan in 2011.

关键词：空气污染；有害空气污染物-放射性核素；国家排放标准；2012

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This study was conducted in Bakersfield, CA to investigate the emissions sources and chemistry controlling aerosol production in the southern San Joaquin Valley Air Basin (SJVAB) a region that is current ly out of compliance with ambient air quality standards for ozone and particulate matter. The primary objective was to invest igate the contributions of various sources to organic aerosol (OA) and the formation pathways of secondary organic aerosol (SOA) and subsequently provide insights into effective control strategies to reduce air pollution. An in-situ thermal desorption aerosol gas chromatography-mass spectrometry (TAG) instrument, equipped with a pm2.5 sharp-cut cyclone, was deployed to measure organic species in both gas and particle phases during the California at the Nexus of Air Quality and Climate Change (CalNex) measurement campaign from May 31st through June 27th, 2010. More than 100 compounds were quantified, including alkanes, polycyclic aromatic hydrocarbons (PAHs), branched PAHs, acids, furanones, and oxygenated compounds, which provided a large set of organic species for the investigation of SOA format ion through comparison between modeled and measured gas/part icle partitioning of Known SOA tracers and OA source apportionment through positive matrix factorization (PMF) analysis.

关键词：空气污染；有机气溶胶；标记化合物；每小时原位定量

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The SunShot Incubator project is a significant effort of the U.S. Department of Energy (DOE) to partner with U.S. industry to accelerate the commercialization of solar energy systems that can meet aggressive cost and capacity goals by the end of the decade. Specifically, the SunShot Incubator projects fund technologies with a disruptive potential to reach the DOE SunShot Initiative goal of an unsubsidized cost-competitive levelized cost of energy by the end of the decade. This is estimated to be approximately 6 cents/kW-hr at utility scale.

关键词：太阳能；低成本；磁悬浮定日镜；技术报告

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Norway, the largest holder of natural gas and oil reserves in Europe, provides much of the oil and gas consumed on the continent. In fact, Norway was the second largest exporter of natural gas in the world after Russia, and the seventh largest exporter of oil. In 2010, crude oil, natural gas, and pipeline transport services accounted for almost 50 percent of Norway's exports revenues, 21 percent of GDP, and 26 percent of government revenues according to the Norwegian Petroleum Directorate (NPD). Although Norway's oil production peaked in 2001 at 3.4 million barrels per day (bbl/d) and declined to 2.0 million bbl/d in 2011, natural gas production has been steadily increasing since 1993, reaching 3.6 trillion cubic feet (Tcf) in 2011. Hydropower is the principal source of Norway's electricity supply at 95 percent, while only 4 percent comes from conventional thermal sources, followed by 1 percent from other renewables, namely biomass and waste and wind. In June 2012, government officials from Norway, Germany, and the United Kingdom (UK) confirmed their plans for subsea electric power interconnects between their countries. The Norway-UK cable connection is slated for completion in 2020 while the Norway-Germany cable is to be completed in 2018; their purpose is to strengthen the northern European electricity grid and increase supply security.

关键词：石油生产;石油消费量;二氧化碳排放量;生物质能

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Cofiring of biomass and coal at electrical generation facilities is gaining in importance as a means of reducing fossil fuel consumption, and more than 40 facilities in the United States have conducted test burns. Given the large size of many coal plants, cofiring at even low rates has the potential to utilize relatively large volumes of biomass. This could have important forest management implications if harvest residues or salvage timber are supplied to coal plants. Other feedstocks suitable for cofiring include wood products manufacturing residues, woody municipal wastes, agricultural residues, short-rotation intensive culture forests, or hazard fuel removals. Cofiring at low rates can often be done with minimal changes to plant handling and processing equipment, requiring little capital investment. Cofiring at higher rates can involve repowering entire burners to burn biomass in place of coal, or in some cases, repowering entire powerplants. Our research evaluates the current status of biomass cofiring in North America, identifying current trends and success stories, types of biomass used, coal plant sizes, and primary cofiring regions. We also identify potential barriers to cofiring. Results are presented for more than a dozen plants that are currently cofiring or have recently announced plans to cofire.

关键词：生物质能；煤炭；化石燃料；森林；燃油消耗

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