关键词：生物传感器；神经细胞；人类胚胎干细胞；毒素和抗毒素
摘 要：The purpose of this project is to develop in vitro, cell based biosensors for environmental toxins. By using ArunA s neural cell lines derived from both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), our goal is to provide a human neural cell based biosensor that is a more biologically relevant model of human physiology. This report describes the progress made in these major areas: (1) directed differentiation of hESC- and hiPSC-derived neural progenitor cells into dopaminergic neurons; (2) directed differentiation of hESC- and hiPSC-derived neural progenitor cells into astrocytes; (3) cell culture medium development for the maintenance and differentiation of ArunA s neural cell lines as sensor elements for neurotoxicity; and (4) label-free, adhesive signature-based microfluidic cell separation.