关键词：片段分配；RNA-Sq测序；高通量测序实验；表达生成模型；期望最大化算法
摘 要：we present our model for ambiguous fragment assignment for RNA-Seq, which includes the most comprehensive set of parameters of any model introduced to date, as well as various methods we have explored for scaling our optimization procedure. These methods include the use of an online EM algorithm and a distributed EM solution implemented on the Spark cluster computing system. Our advances have resulted in the first efficient solution to the problem of fragment assignment in sequencing.