关键词：卷烟烟气；NNK；B[a]P；联合作用；致突变性
摘 要：以苯并[a]芘(B[a]P)、甲基亚硝胺吡啶基丁酮(NNK)为目标化合物,设置卷烟烟气冷凝物(CSC)的浓度为0(CK),0.005,0.010,0.015,0.020 mg/mL；NNK的浓度为0(CK),0.005,0.010,0.020,0.040 mg/mL；B[a]P的浓度为0(CK),0.00025,0.00050,0.00075,0.00100 mg/mL,研究了NNK,B[a]P和CSC的联合致突变性.结果表明:①CSC,NNK和B[a]P均可诱导鼠伤寒沙门氏菌TA98,TA100发生突变,且剂量-效应明显.②B[a]P与CSC联合呈相加作用；B[a]P与NNK联合高剂量呈弱相加作用；CSC与NNK联合低剂量呈弱相加作用.③在不同情况下,三者联合作用致TA98呈弱相加作用.综上,NNK和B[a]P在卷烟烟气基质中的联合致突变性呈现量效、时效和先后作用顺序的复杂性.