关键词：医药；P53-H175；P53-W248；细胞增殖；细胞周期；细胞迁移
摘 要：Objective:p53,a tumor suppressor gene,is one of the hotspots in the world of the biomedical field.Mutation of p53 gene,which is found in approximately 50％ of human cancers,is a key event in carcinogenesis.This project aims to investigate the new characteristics of two p53 mutants,p53-W248 and p53-H175,in MCF-7 cells,so as to provide the experimental basis for understanding the functional alternations of mutant p53.Methods:In this study,MCF-7 cells transfected with p53-H175 or p53-W248 plasmids were used as experimental group and the MCF-7 cells transfected wild type p53 plasmid were used as control group.Then the biological effects at the cellular level were investigated using 3-(4.5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay,flow cytometry analysis and cell scratch test.Results:MTT assay showed that p53-W248 might promote cell proliferation in MCF-7 cells.The results of flow cytometry indicated that no significant effect on cell cycle progression and cell apoptosis by p53-H175 or p53-W248 in cells.The cell scratch test showed that p53-H175 could increase the ability of cell migration.Conclusion:p53-H175 could lead to the promotion of tumor cell migration,while p53-W248 may promote tumor cell proliferation,p53-H175 and p53-W248 might have acquired some new characteristics of oncogenes.