关键词：导电性聚合物；聚吡咯；生物医学
摘 要：Nanocomposites based on the integration of conducting polymer, polypyrrole (PPy) with other nanostructured-materials viz. carbon nanotubes (CNT) and gold nanoparticles (GNP) to construct a suitable host matrix for the functionalization of enzymes hold a great promise for the development of biosensors for the measurement of oxidative stress. Themitochondrial respiratory chain is an important source for the production of cellular reactive oxygen species (ROS) and reactive nitrogen species (RNS), causing a poptosis by the release of cytochromec(cyt c) from mitochondria. Therefore, the quantification of various biomarkers of oxidative stress could be of great importance in the clinical diagnosis and therapeutic research. This chapter presents the bio-functionalization of nanocomposites with enzymes for electrochemical biosensing of oxidative stress biomarkers viz. superoxide, nitricoxide, nitrite, nitrate, cysteine, and cyt c. The synergistic effect of the nanocomposites enhanced the direct electron transfer and catalytic activity of enzymes by providing a well ordered, stable and large surface for the functionalization without affecting their biological activity.