关键词：抗菌药物;细菌;创伤和损伤;攻击;细菌性疾病;杀菌剂;细胞(生物学);主机(生物学);假设;传染病;交互作用;范围(极端);耐药性;安全;治疗;产量;食肉细菌;生物防除;伤口感染
摘 要：Disease-causing microorganisms that have become resistant to drug therapy are an increasing cause of burn, wound, blast and bone infections, with many traditional antimicrobial agents becoming ineffective. Our main hypothesis is that predatory prokaryotes could serve as a novel therapeutic agent to control wound-related bacterial infections. In a previous study, we confirmed that predatory bacteria Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are able to prey on a wide range of pathogens including bacteria isolated from Wounded Warriors. The aim of this proposal is to address key questions regarding the safety and efficacy of predatory bacteria and investigating predator prey interactions and resistance. Using enrichment culturing techniques we have verified that no genetically stable predation resistant phenotype developed in K. pneumoniae and A. baumannii host cells following sequential predation by B. bacteriovorus. Furthermore, sequential predation by M. aeruginosavorus also did not yield host resistance. Our data also confirmed that the predators do not breach their host specificity and attack previously resistant bacteria. Additional enrichment experiments did not produce predators which exhibit enhance predation on a selected host but did yield predators which were acclimated to attack at elevated temperatures.