关键词：非裔美国人;细胞胞质杂种细胞;脱氧核糖核的酸;线粒体,线粒体dna测序;前列腺癌
摘 要：We are examining the hypothesis that mitochondrial inheritance plays a significant role in aggressiveness of prostate cancer in African Americans. In the first year of the project we have identified 2,000 noncancerous tissues samples from African American men with prostate cancer and we have extracted DNA from approximately 1,500 of them to date. We have validated a robust new DNA sequencing technique developed by our collaborator using single amplicon long-range PCR that permits deep coverage (10,000-20,000X on average) of the mitochondrial genome. We have sequenced 549 samples fully thus far. Mapping of DNA variants in our sequenced genomes to mitochondrial genes has begun and the initial data is presented here. After months of testing, we determined that the ethidium bromide methodology for generating prostate cancer cell line cybrids was not effective and we have instead decided to use the Rhodamine-6-G procedure. Thus far the PC3 and PNT1A cell lines are responding well to this method and we expect to produce cybrids within the next month. We have begun testing additional mitochondrial cell lines in case the LNCaP cell cybrid generation is not successful.