关键词：海豚(哺乳动物);干细胞;脂肪组织,细胞(生物学);细胞学;脂肪;创伤和损伤
摘 要：Described herein is the final technical information pertaining to a multi-year effort to determine and characterize the use of adipose (fat)- derived stem cells in the treatment of epidermal (skin) wounds. Adipose tissue was successfully harvested from the nuchal fat pad of six Atlantic Bottlenose dolphins via liposuction; cells released during the digestion of the adipose tissue were analyzed for cytology, assayed for the total number of colony- forming cells, expanded in culture, differentiated into multiple cell lineages, analyzed for stem cell surface markers and stem cell related genes. Cultured cells were also cryogenically frozen for autologous and allogeneic cell therapy treatment of dolphin skin wounds. Stem cells were incubated with white blood cells from non-donor dolphins and were shown to be non-immunogenic. Injection of stem cells along the wound axis into skin wounds of dolphins display a more rapid healing than carrier solution alone.