关键词：生物标志物;临床医学;文化;人类;图像;乳酸;代谢;模型;分子;前列腺癌,前列腺;光谱;合作;组织(生物学)
摘 要：The overall objective of our synergistic team was to develop and apply a novel NMR-compatible model of human prostate cancer (PCa) to identify new hyperpolarized molecular imaging biomarkers for improved clinical management of PCa. We demonstrated that thin, precision-cut prostate tissue slice cultures (TSCs) could be maintained in an NMR-compatible bioreactor and that hyperpolarized 13C spectroscopy could be employed to study real-time metabolism of normal and malignant tissues. The similarity of metabolism in TSCs compared to humans validates this new preclinical model and for the first time provides a representative experimental model that could be widely adopted by others to improve imaging modalities for prostate cancer. Our results suggest that hyperpolarized 13C lactate may serve as a biomarker of prostate cancer.