关键词：医疗服务；肽水解酶；核糖核酸；蛋白酶
摘 要：A platform technology to prepare enzyme sensitive nanomedicine (ESNM), which integrates the imaging and treatment capability, has been developed to report and cure diseases. ESNM is prepared with multiple layers of polyelectrolytes, sequentially assembled on an inert gold nanoparticle, using alternating charged polymers. Potentially the release kinetics could be controlled by varying polyelectrolytes with different properties, such as length, ionic strength and bond stability. Upon incubation with cells, the assembled ESNMs are taken up efficiently. The tight packing and layered assembly of the quenched polyelectrolytes slow subsequent intracellular degradation, and then result in a prolonged intracellular fluorescence signal and therapeutic effect for up to 3 weeks with no noticeable toxicity.