关键词：组合逻辑；大肠杆菌；可伸缩；医疗器械
摘 要：Available to synthetic biologists are a wide range of genetic devices. Many of these devices are able to either sense or alter local conditions. The ability to sense a multitude of inputs combined with diverse outputs could enable engineered organisms that interact with their environment in new and complex ways. Currently the complexity of such systems has been limited by our ability to integrate several inputs into a desired output.Simple combinational logic functions, containing 1 to 3 logic gates, have been constructed in Escherichia coli, but more complex logic networks are needed to fully exploit the opportunities presented by these sensors and actuators. Use of genetic logic gates is constrained by the specific molecular interactions that are used to implement each gate. These interactions involve diffusible molecules that can move within the cytoplasm of the cell and therefore are not spatially separated from other gates. To make larger logic blocks, sets of gates that use unique molecular interactions with minimal crosstalk are required.