关键词：爆炸；眼；创伤和损伤；细胞凋亡
摘 要：In year 1 of this project, we determined whether Compound 49b, a novel beta-adrenergic receptor agonist, could prevent increased inflammatory and apoptosis proteins in mice after exposure to ocular blast. Eyes from C57/BL6 mice were exposed to a blast of air from a paintball gun at 26psi. Eyes were collected at 4, 24, and 72 hours after blast exposure. In a subset of mice, Compound 49b eye drops (1mM dose) were applied within 4 hours, 24 hours, or 72 hours after blast. Three days after exposure to blast, all mice were sacrificed. One eye was used for protein analyses of TNF, IL-1, Bax, Bcl-xL, caspase 3, and cytochrome C. The other eye was used for TUNEL labeling of apoptotic cells, which were co-labeled with NeuN to stain for retinal ganglion cells. We found that ocular exposure to 26psi of air pressure led to a significant increase in both inflammatory and apoptotic proteins. When Compound 49b was applied within 4 or 24 hours after blast, it mitigated the increase in inflammatory and apoptotic proteins. Ocular blast produces a significant increase in inflammatory and apoptotic proteins in the retina, specifically in retinal ganglion cells. These proteins are reduced after treatment with a topical betaadrenergic receptor agonist. This data suggests local application of beta-adrenergic receptor agonists may be protective in ocular blast.