用于及早发现骨转移性乳腺癌的生物相容性,生物可降解和酶裂解的MRI造影剂
Biocompatible, Biodegradable, and Enzymatic-Cleavable MRI Contrast Agents for Early Detection of Bone Metastatic Breast Cancer
关键词:骨头;乳腺癌;磁共振成像;医药
摘 要:This project proposes to design and test a novel peptide-based MRI contrast agent for early detection of bone metastasis from breast cancer. The proposed imaging agent is consist of bone targeting moiety of Asp8 and MRI imaging moiety of DOTA(Gd) with a cathepsin K cleavable peptide spacer. A solid phase peptide synthesis (SPPS) strategy was used to synthesize peptides. Coupling of the peptide onto DOTA followed by Gd complexation was performed to achieve the proposed imaging agent. Non-targeting and CTSK-insensitive controls were similarly prepared. The obtained contrast agents were characterized in terms of bone specificity, enzymatic degradability and biocompatibility. Preliminary testing of contrast enhanced MR imaging at different time points is ongoing in mice with bone metastasis from breast cancer.