The recent technological advancement in the field of next-generation sequencing (NGS) and cell and molecular biology has given birth to the new era of precision medicine, which offers a platform to analyze the variability in genes, lifestyle, and environment of each person individually. Now, the researchers have found an even better option through cell sequencing, which precedes the advancements of next-generation sequencing. In the past few decades, whole genome sequencing (WGS) and whole transcriptome sequencing (WTS) methods have been applied to single cells to combine DNA, RNA, protein, and chromatin information through a single cell. The transcriptomics technique has enabled researchers to use genomics, proteomics, transcriptomics, and epigenomics to understand the heterogeneity of genetically identical cells in the same tissue. The paradigm-shift from bulk cell sequencing to DR-seq (DNA and mRNA sequencing) and G&T-seq (genome and transcriptome), scM&T-seq (simultaneous single-cell methylome and transcriptome sequencing), scNMTseq (single-cell nucleosome, methylation, and transcription sequencing) enable to broaden the understanding of genotypic and phenotypic heterogeneity by using multi-omics approach. Information from different omics platforms can be integrated through this approach, making available deep insights on the effect of these omics’ layers on the underlying mechanisms of disease processes.

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