关键词：解剖模型;文化;生育;体内分析;注射,注射(医学);模型;致瘤的病毒;卵巢癌;蛋白质
摘 要：The purpose of this study is to determine the functional role of the putative oncogene BORIS in ovarian cancer. The proposed studies will use a newly developed mouse model system to assess the oncogenic potential of BORIS expression in the ovarian surface epithelium, alone and in combination with Rb and p53 knockout. In the past year we have accomplished a number of objectives: (i) we have obtained IACUC and Biosafety approvals for the proposed studies, (ii) we designed and constructed a new conditional overexpression construct (iZEG- CTCFL) to drive BORIS expression in mice, (iii) we have designed a TALEN approach to target iZEG-CTCFL to the ROSA26 locus in mice. The constructs have been injected into fertilized eggs from FVB/N mice, and we are currently awaiting founder mice. (iv) we have obtained double conditional knockout mice (Rb, p53) in FVB/N background and bred with FVB/N wild-type mice, obtained F1 and intercrossed to generate stocks of single Rbflox or p53flox mice, (v) Ms. Joanna March, the Teal scholar, has learned how to carry out intrabursal injections and has begun these experiments to meet the study objectives, and (vi) Ms. March has learned how to isolate and culture OSE cells from mice and has begun to isolate and utilize these cultures to meet the study objectives.