报告从政策环境、市场行情、厂商动态、科技研发、国际资讯、行业数据、分析评论等几个方面对医药行业进行了分析评论。

报告从政策环境、市场行情、厂商动态、科技研发、国际资讯、行业数据、分析评论等几个方面对医药行业进行了分析评论。

报告从医药行业事件分析、竞争环境、竞争对手、行业数据等几个方面进行了分析评论。

报告从医药行业事件分析、竞争环境、竞争对手、行业数据等几个方面进行了分析评论。

Mouth ulcers are sores formed in the mucous membrane of the oral cavity. They are noncritical and do not pose serious harm to the health of an individual. Mouth ulcers are formed because of oral injuries or they may arise as a result of an underlying disease. They may be associated with intense or moderate pain. The causes of mouth ulcers are stress, injury, infection, allergy, genetic predisposition, or nutritional deficiencies. The etiology of the disease depends upon the cause, which can be identified and treated upon diagnosis. Ulcers normally heal by themselves in 10-14 days, but medical treatment is usually sought if the condition persists.

Plasma is the liquid component of the blood. It acts as a transport medium for blood cells and platelets. Plasma consists of water, proteins, electrolytes, glucose, clotting factors, and hormones.

Molecular diagnostics is a transformative and dynamic area of diagnostics. The technology has led to advances in treatment and research, enhancing and revolutionizing medical care for a wide range of health conditions. Molecular diagnostics involves the application of molecular biology to medical testing for the analysis of proteomic and genomic biological markers. Currently, hospitals and clinics are using molecular diagnostic tests in the areas of oncology, infectious diseases, pharmacogenomics, and human leukocyte antigen typing.

Cardiac markers are used for risk stratification and diagnosis of patients with chest pain and suspected acute coronary syndrome. Cardiac marker imaging is more effective and less expensive compared to computed tomography and myocardial perfusion imaging. It is a simple technique that provides information to the physician who can then take a decision about opting for an invasive or complicated procedure for treatment. Cardiac marker testing is used in many medical settings, especially in arteriosclerosis, conjunctive heart failure, sudden cardiac arrest, and myocardial infraction.

目的 研究不同辅料(氨基酸、糖类、非离子型表面活性剂)对蛋白药物IgG1单抗热稳定性的影响,探讨辅料与蛋白之间的相互作用,判断辅料与蛋白之间是否存在结合作用。方法 通过差示扫描量热法(differential scanning calorimetry,DSC)获得蛋白的变性温度(Tm)或蛋白某一结构域的变性温度。采用等温滴定量热法(isothermal titration calorimetry,ITC)测定蛋白与不同辅料之间的相互作用。结果 带负电荷的氨基酸可以明显降低IgG1单抗的变性温度(△Tm>9℃),其他辅料作用不明显(△Tm<1℃)。不同pH下辅料对蛋白稳定性的影响不同,在p H=5时带负电荷的氨基酸对IgG1单抗热稳定性的影响大于pH=7时。ITC实验数据显示各种辅料和IgG1单抗的滴定等温线近乎一条直线。结论 IgG1单抗与辅料之间不存在特异性相互作用,带负电荷的氨基酸可以明显降低IgG1单抗的变性温度,应该归功于2者之间的静电相互作用。

目的本研究对TNF家族B细胞激活因子(B cell activating factor to the TNF family,BAFF)的胞外区134~285位氨基酸残基进行克隆,构建原核表达载体pET21a-s BAFF,并进行表达及纯化。方法 以K562细胞系的cDNA为模板,采用PCR扩增s BAFF基因,构建pET21a-s BAFF。在大肠杆菌BL21(DE3)中经IPTG诱导表达sBAFF,超声碎菌,提取包涵体,经Ni2+-IDA亲和层析纯化,复性,采用SDS-PAGE鉴定纯化产物。结果 通过优化确定了目的蛋白适宜的诱导温度和诱导时间。表达的s BAFF相对分子量约为18000,目的蛋白占菌体总蛋白的38.59%,且主要以包涵体的形式存在。经过蛋白复性后有38.14%的sBAFF聚合成了有活性的三聚体,代表错误折叠的二聚体含量极低。结论 建立了成功的复性工艺,目的蛋白经过复性后形成具有活性的三聚体,为进一步研究BAFF的生物学功能以及以BAFF为靶点的自身免疫疾病药物开发奠定了基础。

The objective of the project for the reporting period was to generate a number prostate cancer cell lines that that are either myosin IC isoform A deficient or that constitutively express GFP-myosin IC isoform A. We used shRNA to generate isoform Anegative PC-3 cells and we generated a stable LNCaP cell line that expresses constitutively myosin IC isoform A-GFP. We are now in the process of analyzing the effect of these expression changes on secretion in these cell lines to determine the consequences of isoform A expression changes for the metastatic ability of prostate cancer cells. Using serial cloning of the 5 prime UTR of the human myosin IC gene we have identified a region that is involved in regulatory expression of myosin IC isoform A. We are now in the process of using ChIP assays to identifying the transcriptional elements that bind to this region and induce expression of myosin IC isoform A in prostate cancer cells.

Laboratory studies support the potential for jet fuel to promote noise induced hearing loss. Noise alone induces hearing loss due to loss of hair cells in the cochlea, associated with oxidative stress. Jet fuel toxicity in association with noise may be at least partially explained by increased free radical production and oxidative stress at the cellular level, resulting in hair cell dysfunction and loss. This project combines a physiologically-based pharmacokinetic (PBPK) model to describe jet fuel component concentrations in the cochlea with pharmacodynamic (PD) models of free radical formation in the cochlea by both noise and jet fuel components, and mathematical models to predict the combined impact on hair cell functionality and loss. Further development of this preliminary combined PBPK-PD model of JP-8 induced hearing loss with noise will provide the basis for estimating the potential risk to humans exposed to the same chemical and sound scenarios in occupational settings.